PROMOTER OF GENE SPECIFICALLY EXPRESSED IN RICE CALLUS AND USES THEREOF

摘要

PURPOSE: A promoter of gene specifically expressed in rice callus is provided to be very useful in stably mass expressing, particularly, the protein which has the medical or biological activity originated from the eukaryote, in rice callus. CONSTITUTION: A rice callus specific promoter is composed of a base sequence of Sequence no. 1. A recombined plant expression vector includes said promoter. An isolation method of the callus tissue specific promoter comprises a step of manufacturing rice 3' tiling 135k microarray by designing 4 probes of the length of 60 bp which moves by 30 bp from 3'-terminal to the 5' direction of each gene originated from rice and fixing to a microarray; a step of synthesizing cDNA with RNA isolated upon rice tissues as a template and hybridizing to the manufactured microarray; a step of identifying genes expressed specifically in callus than in other tissues based on the hybridization result; and a step of isolating the promoter of the identified genes.