摘要 |
PROBLEM TO BE SOLVED: To provide a method which allows selection of living cells, with increased rates of reproduction and specific metabolic properties, in a liquid or semi-solid medium.SOLUTION: The method for culturing cells of non-floating state, includes a step of preparing the culture medium, a flexible tube containing a surface on which the living cells can grow, and multiple clamp systems capable of open and closed positions which are positioned so as to divide the tube into: (i) an upstream region containing unused culture medium; (ii) a downstream region containing used culture medium; and (iii) a culture chamber region arranged between the upstream and downstream regions for culturing the cells on the surface. |