发明名称 |
Method for tissue sample fixation |
摘要 |
An aldehyde fixative solution at a first temperature is caused to contact a tissue sample for a first time period, additionally an aldehyde fixative solution is caused to contact the tissue sample at a second temperature higher than the first temperature for a second time period. The first time period typically ranges from about 15 minutes up to about 4 hours, and the first temperature typically is from greater than 0°C to at least 15°C. The second temperature typically is from greater than about 22°C to about 55°C, and the second time period ranges from about 1 hour to about 4 hours. Using this process, improved tissue morphology and IHC staining as well as superior preservation of post-translation modification signals have been accomplished in approximately 4 hours compared to 24 hours for room temperature protocols, and more even morphology and antigen preservation are observed. |
申请公布号 |
AU2012216987(A1) |
申请公布日期 |
2013.07.11 |
申请号 |
AU20120216987 |
申请日期 |
2012.02.17 |
申请人 |
VENTANA MEDICAL SYSTEMS, INC. |
发明人 |
OTTER, MICHAEL;PIERSON, ABBEY;CHAFIN, DAVID;ROBERTS, ESTEBAN |
分类号 |
G01N1/30 |
主分类号 |
G01N1/30 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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