发明名称 |
Immobilized nucleic acid complexes for sequence analysis |
摘要 |
Provided are methods for sequencing a nucleic acid that include fixing a template to a surface through a template localizing moiety and sequencing the nucleic acid with a sequencing enzyme, e.g. a polymerase or exonuclease. The sequencing enzyme can optionally be exchanged with a second sequencing enzyme, which continues the sequencing of the nucleic acid. The template localizing moiety can optionally anneal with the nucleic acid and/or associate with the sequencing enzyme. Also provided are compositions comprising a nucleic acid fixed to a surface via a template localizing moiety, and a first sequencing enzyme, which can sequence the nucleic acid and optionally exchange with a second sequencing enzyme present in the composition. Compositions in which a template localizing moiety is immobilized on a surface are provided. Compositions for sequencing reactions are provided. Also provided are sequencing systems comprising reaction regions in which or near which template localizing moieties are immobilized.
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申请公布号 |
US8481264(B2) |
申请公布日期 |
2013.07.09 |
申请号 |
US20090562690 |
申请日期 |
2009.09.18 |
申请人 |
BJORNSON KEITH;BIBILLO AREK;CHRISTIANS FRED;TRAVERS KEVIN;EMIG ROBIN;PACIFIC BIOSCIENCES OF CALIFORNIA, INC. |
发明人 |
BJORNSON KEITH;BIBILLO AREK;CHRISTIANS FRED;TRAVERS KEVIN;EMIG ROBIN |
分类号 |
C12Q1/68;C07H21/04;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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