发明名称 |
Method for chromogenic detection of two or more target molecules in a single sample |
摘要 |
The present invention provides a method and kit for detection of two or more target molecules in a single tissue sample, such as for gene and protein dual detection in a single tissue sample. Methods comprise treating a tissue sample with a first binding moiety that specifically binds a first target molecule. Methods further comprise treating the tissue sample with a solution containing a soluble electron-rich aromatic compound prior to or concomitantly with contacting the tissue sample with a hapten-labeled binding moiety and detecting a second target molecule. In one example, the first target molecule is a protein and the second is a nucleic acid sequence, the first target molecule being detected by immunohistochemistry and the second by in situ hybridization. The disclosed method reduces background due to non-specific binding of the hapten-labeled specific binding moiety to an insoluble electron rich compound deposited near the first target molecule.
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申请公布号 |
US8481270(B2) |
申请公布日期 |
2013.07.09 |
申请号 |
US200913059274 |
申请日期 |
2009.08.21 |
申请人 |
GNIEWEK RICHARD;FARRELL MICHAEL;NITTA HIROAKI;LEHRKAMP MEGAN;KOSMEDER JEROME;KELLY BRIAN DANIEL;GROGAN THOMAS;GAIRE FABIEN;PADILLA MARY;BIENIARZ CHRISTOPHER;VENTANA MEDICAL SYSTEMS, INC. |
发明人 |
GNIEWEK RICHARD;FARRELL MICHAEL;NITTA HIROAKI;LEHRKAMP MEGAN;KOSMEDER JEROME;KELLY BRIAN DANIEL;GROGAN THOMAS;GAIRE FABIEN;PADILLA MARY;BIENIARZ CHRISTOPHER |
分类号 |
C12Q1/68;G01N33/53;G01N33/58 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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