PROCEDURE FOR IDENTIFYING THE SPECIES AND STRAIN OF LACTOBACILLUS USING THE GENES OF TYPE II TOXIN-ANTITOXIN SYSTEMS OF THE SUPERFAMILIES RELBE AND MAZEF

摘要

The invention relates to biological engineering, in particular to medical microbiology, and also to the production of cultures for sour milk food products and can be used for identifying Lactobacillus strains in the human microbiota, in food products and in a production line for said products. The invention comprises a novel procedure for identifying phylogenetic groups and individual species of Lactobacillus, Lactobacillus strains of the species Lactobacillus rhamnosus, which procedure is based on a combination and polymorphism of genes of type II toxin-antitoxin (TA) systems of the superfamilies MazEF and RelBE. Oligonucleotides matching such genes can be used for the characteristics of the strain, for identifying the strain diversity in the ecological niche under investigation. Various polymerase chain reaction procedures (multilocus polymerase chain reaction, polymerase chain reaction in real-time mode, etc.) with subsequent sequencing, including using the MiSeq system (Illumina, USA) using proposed pairs of oligonucleotides can be presented as a diagnostic procedural approach for determining a strain under investigation in clinical samples or for molecular tracing of the strain in commercial preparations.