METHOD FOR PRODUCING RETINAL PIGMENT EPITHELIAL CELL

摘要

PROBLEM TO BE SOLVED: To provide a method for highly efficiently producing a retinal pigment epithelial cell.SOLUTION: There is provided a method for producing a retinal pigment epithelial cell, comprising following steps of (1) to (4), wherein (1) a first step of subjecting a pluripotent stem cell to floating culture in a serum-free culture medium containing a Wnt signaling pathway inhibitor to form an aggregate of the pluripotent stem cells, (2) a second step of subjecting the aggregate formed in the first step to floating culture in a serum-free culture medium containing a basement membrane preparation, (3) a third step of subjecting the aggregate cultured in the second step to floating culture in a serum-containing culture medium, and (4) a fourth step of subjecting the aggregate cultured in the third step to floating culture in a serum-free culture medium or a serum-containing culture medium each containing a substance capable of acting on the Wnt signaling pathway (wherein the serum-free culture medium and the serum-containing culture medium do not contain any substance acting on the Sonic hedgehog signaling pathway).