| 摘要 |
PROBLEM TO BE SOLVED: To provide a method capable of highly efficiently producing a retinal tissue.SOLUTION: There is provided a method for producing a retinal tissue, and comprising following steps of (1) to (3), wherein, (1) a first step of subjecting a pluripotent stem cell to floating culture in a serum-free culture medium containing a Wnt signaling pathway inhibitor to form an aggregate of the pluripotent stem cells, (2) a second step of subjecting the aggregate formed in the first step to floating culture in a serum-free culture medium containing a basement membrane preparation, and (3) a third step of subjecting the aggregate cultured in the second step to floating culture in a serum-containing culture medium. |
