HN PROTEIN OF NEWCASTLE DISEASE VIRUS AND THE DIAGNOSTIC COMPOSITION FOR NEWCASTLE DISEASE CONTAINING THE SAME

摘要

PURPOSE: An HN protein of Newcastle disease virus (NDV) and an NDV antigen diagnostic liquid containing the same for a hemagglutination inhibition (HI) method are provided to quickly and cheaply produce the NDV antigen diagnostic liquid without contamination or disease infection. CONSTITUTION: A method for preparing a recombinant baculovirus expression vector comprises the steps of: isolating virus genome RNA from NDV Lasota strain; synthesizing cDNA of HN protein of NDV protein using a reverse primer of sequence number 2 and RNA; performing PCR of cDNA using a forward primer of sequence number 1, and a reverse primer of sequence number 2 to synthesize DNA; treating the synthesized DNA with Xba1 and Hind III, and extracting DNA fragments; and inserting the DNA fragment into a multicloning site of pFastBac TM 1 expression vector with baculovirus polyhedron promoter. [Reference numerals] (AA) Novel method; (BB) Conventional method; (CC) Normal cells