MARKERS FOR GANODERMA DISEASE DIAGNOSIS AND A USE OF THEREOF

摘要

Abstract MARKERS FOR GANODERMA DISEASE DIAGNOSIS AND A USE OF THEREOFThe invention relates to a marker comprising a sequence as set forth in SEQ. ID 1 or a functional fragment thereof to diagnose Ganoderma disease in palm plants in particular oil palm plants. The invention also relates to a marker comprising a sequence as set forth in SEQ. ID 2 or a functional fragment thereof to diagnose Ganoderma disease in palm plants in particular oil palm plants. The invention also provides the use of one or a combination thereof of markers comprising a sequence as set forth in SEQ. ID 1 or functional fragment thereof and markers comprising a sequence as set forth in SEQ. ID 2 or functional fragment thereof to diagnose Ganoderma disease in palm plants in particular oil palm plants. The invention also provides a method of diagnosing Ganoderma disease in palm plants in particular oil palm plants wherein the method includes the steps of (a) preparing a PCR reaction mixture with selected markers for detecting Ganoderma disease and (b) running a PCR process with the mixture from step (a) with an annealing temperature between the range of 50 °C to 61 °C. The markers used is one or a combination thereof of markers comprising a sequence as set forth in SEQ. ID 1 or functional fragment thereof and markers comprising a sequence as set forth in SEQ. ID 2 or functional fragment thereof and wherein the diagnosis of a palm plant infected or has a risk of being infected by the Ganoderma disease is determined by the absence of gene of interest.FIGs. 1, 2, 3 and 4