摘要 |
<p>PURPOSE: DNA oligomer is provided to enhance knock-down effect by interfering with a stable DNA, to regulate secretion of various interleukins, and to regenerate skin in wound by regulating TGF-beta 3 expression level. CONSTITUTION: A method for reducing TGF-beta 3 expression level in cultured cells, tissues, or organism comprises a step of preparing a DNA oligomer with 10 or more base pairs which complementarily binds to TGF-beta 3. The DAN oligomer contains 3'-aaatttcgatttttccgg-5'(DNA strand which is complementary to upstream sequence), 3'-cgtatgacatgtacttataa-5'(DNA strand which is complementary to midstream sequence), and 3'-gatacctccttaagatta-5'(DNA strand which is complementary to downstream sequence). The DNA oligomer is prepared by synthesis of a PCR primer. [Reference numerals] (AA) Front sequence(underline = complementary strand, 3'---5' = antisense DNA sequence); (BB) Middle sequence(underline = complementary strand, 3'---5' = antisense DNA sequence); (CC) Rear sequence(underline = complementary strand, 3'---5' = antisense DNA sequence)</p> |