摘要 |
The disclosure provides transaminase polypeptides capable of converting the substrate, 2-(3,4-dimethoxyphenethoxy)cyclohexanone to the trans diastereomer product (1R,2R)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine in at least a 2:1 diastereomeric ratio relative to the cis diastereomer (1R,2S)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine. The disclosure also provides polynucleotides, vectors, host cells, and methods of making and using the transaminase polypeptides in processes for preparing (1R,2R)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine and its analogs, which can product compounds can be further used to prepare the aminocyclohexylether compound, (3R)-1-[(1R,2R)-2-[2-(3,4-dimethoxyphenyl)ethoxy]cyclohexyl]pyrrolidin-3-ol, which is an ion channel blocker.
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申请人 |
LIMANTO JOHN;BEUTNER GREGORY;GRAU BRENDAN;JANEY JACOB;KLAPARS ARTIS;ASHLEY ERIC;STROTMAN HALLENA R.;TRUPPO MATTHEW D.;HUGHES GREGORY;CABIROL FABIEN L.;GOHEL ANUPAM;COLLIER STEVEN J.;LIANG JACK;MOCK MARISSA;MUNDORFF EMILY;NOVICK SCOTT;SMITH DEREK;CODEXIS, INC. |
发明人 |
LIMANTO JOHN;BEUTNER GREGORY;GRAU BRENDAN;JANEY JACOB;KLAPARS ARTIS;ASHLEY ERIC;STROTMAN HALLENA R.;TRUPPO MATTHEW D.;HUGHES GREGORY;CABIROL FABIEN L.;GOHEL ANUPAM;COLLIER STEVEN J.;LIANG JACK;MOCK MARISSA;MUNDORFF EMILY;NOVICK SCOTT;SMITH DEREK |