| 摘要 |
<p>PURPOSE: A DNA oligomer is provided to enhance knock-down effect by interfering with stable DNA and to regulate various cAMP-dependent signal transduction systems, to suppress cancer cell, and to maintain normal cell cycle. CONSTITUTION: A method for reducing Ras protein expression level in a cultured cell, a tissue, or organism comprises a step of preparing a DNA oligomer with 10 or more base pairs, which complementarily binds to Ras mRNA. The DNA oligomer contains 3'-gccccggctcttggcctt-5'(DNA strand which is complementary to upstream sequence), 3'-gacactcgtcctggtccg-5'(DNA strand which is complementary to midstream sequence), and 3'-aacctcggcaaaggcacc-5'(DNA strand which is complementary to downstream sequence) of 18 base pairs. The DNA oligomer is prepared by synthesis of a PCR primer. [Reference numerals] (AA) Front sequence(underline = complementary strand, 3'---5' = antisense DNA sequence); (BB) Middle sequence(underline = complementary strand, 3'---5' = antisense DNA sequence); (CC) Rear sequence(underline = complementary strand, 3'---5' = antisense DNA sequence)</p> |
