| 摘要 |
<p>PURPOSE: A DNA oligomer is provided to enhance knock-down effect by interfering a stable DNA, to suppress various cancers, and to control cell proliferation and division by regulating cyclin dependent kinase 1 expression level. CONSTITUTION: A method for reducing cyclin dependent kinase 1 expression level in cultured cells or tissues and organisms or application thereof comprises a step of preparing a DNA oligomer with 10 or more base pairs, which complementarily binds to cyclin dependent kinase 1 mRNA. The DNA oligomer contains 3'-caaactttgacgagcgtg-5'(DNA strand which is complementary to upstream), 3'-catttaatattttaacat-5' (DNA strand which is complementary to midstream sequence), and 3'-cagaaggtgtgtatgaaa-5'(DNA strand which is complementary to downstream sequence). The DNA oligomer is prepared by synthesis of a PCR primer. [Reference numerals] (AA) Front sequence(underline = complementary strand, 3'---5' = antisense DNA sequence); (BB) Middle sequence(underline = complementary strand, 3'---5' = antisense DNA sequence); (CC) Rear sequence(underline = complementary strand, 3'---5' = antisense DNA sequence)</p> |
