发明名称 |
INDUCED SPORULATION SCREENING METHOD |
摘要 |
<p>The present invention relates to a method of screening for an improved enzyme variant, said method comprising the steps of: (i) providing a recombinant host cell capable of sporulating comprising a polynucleotide encoding a sporulation factor and a polynucleotide encoding an enzyme variant which are operably linked to an inducible promoter; (ii) culturing the host cell under conditions suitable to induce the formation of spores; (iii) culturing the spores obtained in step (ii) in a medium containing a substrate for the enzyme variant; and (iv) determining the activity of the enzyme variant.</p> |
申请公布号 |
EP2606146(A1) |
申请公布日期 |
2013.06.26 |
申请号 |
EP20110748349 |
申请日期 |
2011.08.18 |
申请人 |
NOVOZYMES A/S |
发明人 |
BJOERNVAD, MADS;PEDERSEN, POUL ERIK;MALTEN, MARCO |
分类号 |
C12Q1/02 |
主分类号 |
C12Q1/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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