发明名称 |
HELICASE DEPENDENT ISOTHERMAL AMPLIFICATION USING NICKING ENZYMES |
摘要 |
The present invention relates to a method for amplifying a template nucleic acid, wherein the method comprises amplifying said template nucleic acid using the helicase dependent amplification (HDA) reaction in the presence of a nicking endonuclease, and wherein said template nucleic acid comprises a sequence recognized by said nicking endonuclease or a sequence recognized by said nicking endonuclease is introduced into the template nucleic acid during the HDA reaction. The invention further pertains to a kit for amplifying a nucleic acid, comprising a nicking endonuclease, a helicase and a DNA polymerase. |
申请公布号 |
EP2606148(A1) |
申请公布日期 |
2013.06.26 |
申请号 |
EP20110749149 |
申请日期 |
2011.08.16 |
申请人 |
QIAGEN GMBH |
发明人 |
KORFHAGE, CHRISTIAN;GROSSHAUSER, GERD;ROTHMANN, THOMAS;HIMMELREICH, RALF |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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