发明名称 |
METHODS FOR THE GENERATION OF MULTISPECIFIC AND MULTIVALENT ANTIBODIES |
摘要 |
The invention provides novel bispecific monoclonal antibodies carrying a different specificity for each binding site of the immunoglobulin molecule and methods for producing novel bispecific monoclonal antibodies carrying a different specificity for each binding site of the immunoglobulin molecule. The antibodies are composed of a single heavy chain and two different light chains, one containing a Kappa constant domain and the other of a Lambda constant domain. The invention provides methods for the isolation of antibodies of different specificities but sharing a common heavy chain. The invention also provides methods for the controlled co-expression of two light chains and a single heavy chain leading to the assembly of monospecific and bispecific antibodies. The invention provides a mean of producing a fully human bispecific and bivalent antibody that is unaltered in sequence and does not involve the use of linkers or other non-human sequences, as well as antibody mixtures of two monospecific antibodies and one bispecific antibody. The invention also provides the means of efficiently purifying the bispecific antibody. |
申请公布号 |
EP2606064(A2) |
申请公布日期 |
2013.06.26 |
申请号 |
EP20110788226 |
申请日期 |
2011.08.16 |
申请人 |
NOVIMMUNE S.A. |
发明人 |
FISCHER, NICOLAS;MAGISTRELLI, GIOVANNI;GUENEAU, FRANCK;RAVN, ULLA;ELSON, GREG |
分类号 |
C07K16/00;C07K16/24;C07K16/28;C07K16/46;C12N15/10 |
主分类号 |
C07K16/00 |
代理机构 |
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代理人 |
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地址 |
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