A method for isolating and purifying of Platycodon gradiflorum fraction with highly purified platycosides

摘要

PURPOSE: An isolation and purification method of platicodi radix fractions including high purity platycoside is provided to simply and economically obtain platicodi radix fractions in short time. CONSTITUTION: An isolation and purification method of platicodi radix fractions including high purity platycoside comprises the following steps: dissolving platicoside ethanol crude extract in ethanol and adding acetone; removing precipitates and concentrating filtrate in order to obtain platicoside ethanol-acetone fraction; obtaining water fraction by dissolving the platicoside ethanol-acetone fraction and concentrating water layer by dividing ethylacetate; obtaining butanol fractions by concentrating the butanol layer after separting the water layer from the butanol layer; and isolation purifying the Platicodi Radix fractions which contains high purity Platicodi Radix platicoside by using reverse phase cartridge chromatography.