发明名称 |
REAL-TIME REDOX SEQUENCING |
摘要 |
Real time redox sequencing methods, devices, and systems are described. Arrays of redox devices comprising one or two electrodes are used to provide sequence information about a template nucleic acid in a polymerase-template complex bound proximate to the electrode(s). A sequencing reaction mixture comprising nucleotide analogs comprising redox labels is introduced to the array of redox devices under conditions of polymerase mediated nucleic acid synthesis. The time sequence of incorporation of nucleotide analogs is determined by electrochemically identifying the redox labels of the nucleotide analogs that are incorporated into the growing strand. |
申请公布号 |
WO2013056241(A3) |
申请公布日期 |
2013.06.20 |
申请号 |
WO2012US60275 |
申请日期 |
2012.10.15 |
申请人 |
PACIFIC BIOSCIENCES OF CALIFORNIA, INC. |
发明人 |
KORLACH, JONAS;TURNER, STEPHEN;SUN, LEI |
分类号 |
C12Q1/68;C12Q1/48;G01N27/26 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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