| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide methods for producing N-terminal derivatives of proteins that is reacted at the N-terminal of a protein or peptide under controlled conditions to produce an N-terminal derivative. <P>SOLUTION: The present invention relates to N-terminal derivatives of proteins in which a polysaccharide, preferably having at least terminal sialic acid units and preferably consisting essentially only of sialic acid units, is reacted at the N-terminal of a protein or peptide under controlled conditions to produce an N-terminal derivative. The controlled conditions include use of acidic pH for the derivatisation step and a higher pH for purification. The derivatives are useful for improving pharmacokinetics and pharmacodynamics of proteins and peptides. <P>COPYRIGHT: (C)2013,JPO&INPIT |
