发明名称 |
Method for Reducing Adapter-Dimer Formation |
摘要 |
Methods are provided for ligating a 3' adapter and a 5' adapter to a target polynucleotide so as to avoid adapter dimer formation. Embodiments of the methods include adding a blocking oligonucleotide after the first ligation in which a 3' adapter is ligated to the target polynucleotide so that the blocking oligonucleotide is capable of hybridizing to excess 3' adapter and the ligated 3' adapter. Subsequently, a 5' adapter is ligated to the target polynucleotide thus avoiding adapter dimer formation. |
申请公布号 |
US2013157869(A1) |
申请公布日期 |
2013.06.20 |
申请号 |
US201113383466 |
申请日期 |
2011.08.31 |
申请人 |
MCREYNOLDS LARRY A.;MUNAFO DANIELA;NEW ENGLAND BIOLABS, INC. |
发明人 |
MCREYNOLDS LARRY A.;MUNAFO DANIELA |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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