| 摘要 |
PURPOSE: Producing high yields of 10-hidroxystearic acid by oleate hydratase is provided to produce high yields of 10-hydroxystearic acid as a source material used for industrial products, and usefully and environmentally friendly applied as a starting material of lactone. CONSTITUTION: A material for producing10-hydroxystearic acid comprises oleate hydratase with the amino acid sequence of sequence number 1 and organic servant. An enzyme is made from Lysinibacillus fusiformis. An organic solvent is ethanol. The Material for producing10-hydroxystearic acid comprises a gene capable of coding oleate hydratase with sequence number 2, and the organic solvent as an active ingredient. Producing 10-hydroxystearic acid comprises bioconversion in the organic solvent by using oleate hydratase to make hydroxyl fatty acid from unsaturated fatty acid, and oleate hydratase and a substrate. The substrate is oleic acid. A process is conducted from PH 6.0 to PH 7.0 and from 25°C to 30°C. |
