| 摘要 |
The invention relates to a method for manipulating, isolating, detecting or amplifying a target nucleic acid in a sample by hybridization with an oligonucleotide-oligocation conjugate, comprising allowing said nucleic acid to react with an oligonucleotide-oligocation conjugate comprising at least A1 and Bj linked together directly or via a linker, wherein. A, is an i-mer oligonucleotides, with i=3 to 50, where Ai is an oligomer with naturally or non naturally occurring nucleobases and/or pentafuranosyl groups and/or native phosphodiester bonds, optionally comprising a marker group. Bj is a j-mer organic oligocation moiety, with j=1 to 50, where B is -HPO3-R1-(NH-R2)n-NH-R3-O-, where R1, R2 and R3 are lower alkylene, identical or different, NH-R2 moieties being identical or different when n is >1; HPO3-R1-CH(X)-R3-O-, where Ri and R3, identical or different, are lower alkylene and X is putrescine, spermidine or spermine residue.
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