摘要 |
The present invention relates to a device for the optical analysis of a sample, also referred to as a microscope, which is provided for a high resolution optical analysis method for detecting fluorescent molecules. The device and the method carried out using the device are designed such that excitation light generated by an excitation light source is directed onto a sample and light emitted by the sample is detected. The device and the method are characterised in that the excitation light is synchronised with the detection process. The device is characterised by having a polarisation device provided for modulating the polarisation of the excitation light by means of a modulation signal, the modulation signal having or consisting of at least one frequency, in particular a pre-determined frequency or a plurality of pre-determined superimposed frequencies, or the modulation signal consisting of a sequence of non-repeated signals. |