发明名称 |
DNA encoding hydantoinase, DNA encoding N-carbamyl-L-amino acid hydrolase, recombinant DNA, transformed cell, method of producing protein, and method of producing optically active amino acid |
摘要 |
The present invention provides a method of producing optically active amino acids from 5-substituted hydantoin by isolating a hydantoinase gene and an N-carbamyl-L-amino acid hydrolase gene involved in an ability to convert 5-substituted hydantoin or N-carbamylamino acid into optically active amino acids from a microorganism of the genus Microbacterium having the above ability and by improving gene amplification and transcriptional and translational activities thereby preparing a recombinant wherein the amount of the desired enzymes produced is increased. The hydantoinase gene is, for example, a DNA encoding for a protein having a hydantoinase activity, which has the nucleotide sequence of SEQ ID NO:1. The N-carbamyl-L-amino acid hydrolase gene is, for example, a DNA encoding for a protein having an N-carbamyl-L-amino acid hydrolase activity, which has the nucleotide sequence of SEQ ID NO:3.
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申请公布号 |
US8460902(B1) |
申请公布日期 |
2013.06.11 |
申请号 |
US20080035525 |
申请日期 |
2008.02.22 |
申请人 |
TAKENAKA YASUHIRO;SUZUKI SHUNICHI;ONISHI NORIMASA;YOKOZEKI KENZO;AJINOMOTO CO., INC. |
发明人 |
TAKENAKA YASUHIRO;SUZUKI SHUNICHI;ONISHI NORIMASA;YOKOZEKI KENZO |
分类号 |
C12N15/09;C12N15/55;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N9/78;C12N9/80;C12N9/86;C12P13/04;C12P13/22;C12P41/00;C12R1/01 |
主分类号 |
C12N15/09 |
代理机构 |
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地址 |
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