发明名称 |
Methods and compositions to detect nucleic acids in a biological sample |
摘要 |
Methods of the invention separate a target nucleic acid from a sample by using at least one capture probe oligonucleotide that contains a target-complementary region and a member of a specific binding pair that attaches the target nucleic acid to an immobilized probe on a capture support, thus forming a capture hybrid that is separated from other sample components before the target nucleic acid is released from the capture support and hybridized to a detection probe to form a detection hybrid that produces a detectable signal that indicates the presence of the target nucleic acid in the sample. Compositions for practicing the methods of the invention include a capture probe oligonucleotide made up a target-complementary region sequence and a covalently linked capture region sequence that includes a member of a specific binding pair.
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申请公布号 |
US8460869(B2) |
申请公布日期 |
2013.06.11 |
申请号 |
US201113087282 |
申请日期 |
2011.04.14 |
申请人 |
BECKER MICHAEL M.;MAJLESSI MEHRDAD R.;GEN-PROBE INCORPORATED |
发明人 |
BECKER MICHAEL M.;MAJLESSI MEHRDAD R. |
分类号 |
C12Q1/68;C07H21/00;C07H21/02;C07H21/04;C12M1/34;C12M3/00;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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