发明名称 |
Esterasegruppe til enantioselektiv fremstilling af fin- og specialkemikalier |
摘要 |
<p>The present invention relates to a polynucleotide consisting of more than 30 nucleotides encoding an enzyme having carboxylesterase [E.C. 3.1.1.1] activity, wherein the coding sequence is selected from the group consisting of (a) a polynucleotide encoding an amino acid sequence as depicted in any one of SEQ ID NOs: 10, 4; 8, and 12; (b) a polynucleotide consisting of or comprising a nucleotide sequence, wherein the nucleic acid sequence is as shown in any one of SEQ ID NOs: 9, 3, 7 and 11; (c) a polynucleotide consisting of or comprising a nucleotide sequence encoding a fragment or derivative of an enzyme encoded by a polynucleotide of (a) or (b), wherein in said derivative one amino acid residue is conservatively substituted compared to the amino acid sequence of (a); (d) a polynucleotide encoding an enzyme having carboxylesterase activity which polynucleotide is at least 66% identical to a polynucleotide encoding an enzyme as shown in any one of SEQ ID NOs: 10, 4, 8 and 12; (e) a polynucleotide consisting of or comprising a nucleotide sequence the complementary strand of which hybridizes under stringent conditions to a polynucleotide as defined in any one of (a) to (b); and (f) a polynucleotide consisting of or comprising a nucleotide sequence being degenerate to the nucleotide sequence of the polynucleotide of (e); or the complementary strand of such a polynucleotide of (a) to (f).</p> |
申请公布号 |
DK2013337(T3) |
申请公布日期 |
2013.06.10 |
申请号 |
DK20070724844T |
申请日期 |
2007.05.03 |
申请人 |
B.R.A.I.N. BIOTECHNOLOGY RESEARCH AND INFORMATIONNETWORK AG |
发明人 |
ECK, JUERGEN;LIEBETON, KLAUS;BORNSCHEUER, UWE;BOETTCHER, DOMINIQUE;LANGER, PETER;BELLUR, ESENORGANIZE SANAYL BOELGESI |
分类号 |
C12N9/18;C07K16/40;C12N5/10;C12N15/55 |
主分类号 |
C12N9/18 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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