METHOD FOR PREPARING BIOSPECIFIC HYDROGEL SORBENT FOR PROTEINASE RECOVERY

摘要

FIELD: medicine.SUBSTANCE: invention describes a method for preparing a biospecific hydrogel sorbent for proteinase recovery by the radical polymerization of an aqueous solution containing ovomucoid 0.1-0.9 wt % of duck egg protein acylated by acryloyl chloride, acrylamide 7.0-12.0 wt % and N,N'-methylenebisacrylamide 0.5-1.1 wt % at room temperature under the action of a redox polymerisation catalyst; the prepared hydrogel is ground and washed in a bicarbonate buffer to remove the reacted compounds completely with the polymerisation conducted in mercaptoacetic acid 0.01-0.12 wt %.EFFECT: more effective use of ovomucoid.1 tbl, 14 ex