| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide methods that can be used to monitor the enrichment processes. <P>SOLUTION: The method (900) for determining an efficiency of target enrichment from a DNA library, includes: the step (901) of adding a negative control sequence and a positive control sequence to the DNA library, or picking a negative control sequence and/or a positive control sequence from the library; the step (902) of determining a pre-capture amount of the negative control sequence and a pre-capture amount of the positive control sequence; the step (903) of performing enrichment of a target sequence from the DNA library using a bait sequence to produce a post-capture library; the step (904) of determining a post-capture amount of the negative control sequence and a post-capture amount of the positive control sequence in the post-capture library; and the step (905) of determining the efficiency of the target enrichment, based on the post-capture amount of the positive control sequence, the post-capture amount of the negative control sequence, the pre-capture amount of the positive control sequence, and the pre-capture amount of the negative control sequence. <P>COPYRIGHT: (C)2013,JPO&INPIT |
