| 摘要 |
FIELD: biotechnologies.SUBSTANCE: versions of delta-12-oleatedesaturase (FAD2) proteins are presented, where one version has an amino-acid substitution in the position corresponding to the position 108 for aspartic acid, and the other one - substitutions in positions corresponding to positions 108 for aspartic acid and 118 for phenyl alanine, in the protein FAD2 of wild type with a sequence SEQ ID NO:4 or SEQ ID NO:8, given in the description. The following are described: nucleic acids, which code the specified proteins; vectors of expression containing the specified nucleic acids; master cells containing the specified vectors. Fragments are proposed, which contain at least 20 nucleotides of the extracted molecule of nucleic acid, containing the specified substitutions, for use as a primer, a probe and/or a marker. A plant is proposed, which creates a high profile of oleic acids in oil of seeds transformed by nucleic acid with a sequence SEQ ID NO:1, or SEQ ID NO:1 and SEQ ID NO:5.EFFECT: invention makes it possible to increase content of oleic acid in seeds of oil-bearing plants.19 cl, 2 tbl, 1 dwg, 3 ex |
