A STABLE CELL LINE ENCODING HUMAN BMP2/7-PTD FOR EFFICIENT OSTEOGENIC INDUCTION AND DEVELOPMENT OF 3 DIMENSIONAL CELL CULTURE USING THE STABLE CELL LINE

摘要

PURPOSE: A cell line which is transformed by a human BMP2/7-PTD expression vector for promoting osteogenesis in human, and a three dimensional cell culture method using the transformed cell line are provided to remarkably reduce BMP protein use, and to effectively create artificial bones by constructing a high quality BMP production system. CONSTITUTION: A human osteogenesis expression vector, pCEP4-rhBMP2/7-PTD, contains a BMP2 gene, a BMP7 gene, and a protein transduction domain(PTD). A method for preparing the expression vector comprises a step of inserting the BMP2 gene into a pCEP4 vector with a CMV promoter, and inserting the BMP7 gene and the PTD. A transformed cell line contains the expression vector.