MOLECULAR ASSAY FOR THE AMPLIFICATION AND DETECTION OF KPC GENES RESPONSIBLE FOR HIGH-LEVEL RESISTANCE TO CARBAPENEM IN GRAM NEGATIVE BACTERIA

摘要

Methods and kits useful for the detection and identification of carbapenem-resistant pathogens harboring carbapenemase-encoding nucleic acids. Said methods comprise PCR amplification of a target region of the beta-lactamase encoding Klebsiella pneumoniae carbapenemase genes (blaKPC) and variants thereof with a primer set comprising SEQ ID Nos: 1 and 2, and variants thereof