METHODS FOR THE PHENOTYPIC DETECTION OF HCV INHIBITOR RESISTANT SUBPOPULATIONS

摘要

Methods and compositions for the efficient and accurate determination of MIsceptibility of a hepatitis C virus (HCV) population to an HCV inhibitor are provided. In certain aspects, the methods involve introducing into a cell a patient derived segment, wherein the cell or the patient derived segment comprises an indicator nucleic acid that produces a detectable signal that is dependent on the HCV; measuring the expression of the indicator gene in the presence of varying concentn'ltions of the HCV inhibitor: determining a standard curve of susceptibility: comparing the IC95 fold change, slope, or maximum inhibition percentage of the HCV population to that of a control HCV population