| 摘要 |
The present invention provides methods and kits in assessing color change in a series of reaction mixes while providing significant improvement in the amount of non-specific binding. The method provides for a dramatic color change when incorporated into a series of assays or used in an individual reaction. The color change enables quick visual tracking and verification of the addition of reaction components. Further, the color change allows for verification that an instrument is performing correctly in reagent addition during an automated assay. In one embodiment, the reaction mix is initially a yellowish color. Upon adding serum or plasma, the resulting solution undergoes a dramatic color change to blue-purple, allowing for easy visual verification. Color change depends upon pH shifts in the sample preparation upon addition of serum or plasma. The method is useful whenever two or more reagents are combined and confirmation of the combination is required. |
