METHOD FOR PREPARING SUGAR CHAINS FROM ANTIBODIES

摘要

A method allowing easy and quick preparation of sugar chains of antibodies is provided. Sugar chains are cut off from the antibodies in a state where the antibodies are kept being bonded to an absorbing materials, such as the Protein A or the Protein G, Then, the cut off sugar chains are captured to sugar-chain-capturing solid-phase carriers having a functional group that forms a stable bonding with the sugar chains by reacting specifically with the aldehyde groups of the sugar chains. Then, the captured sugar chains are re-released. Sugar chain samples suitable for mass spectrometry, chromatography, or electrophoresis can be prepared easily and quickly by the present method.