| 摘要 |
PURPOSE: A method for preparing glutaric acid from lysine using a recombinant microorganism is provided to ensure significant productivity of the glutaric acid from the lysine and to be used in technology for inexpensively producing a large amount of glutaric acid. CONSTITUTION: A recombinant microorganism for preparing glutaric acid comprises: a step of preparing an expression vector containing one or more genes among genes encoding lysine 2-monooxygenase(DavB), delta-aminovaleramidase(DavA), 5-aminovalerate aminotransferase(DavT), and glutarate semialdehyde dehydrogenase(DavD); a step of transforming one or more expression vectors into a microorganism and preparing a recombinant microorganism containing DavB, DavA, DavT, and DavD genes. The microorganism is E. coli. The expression vector includes: a recombinant vector which is prepared by inserting genes encoding DavB and DavA into an expression vector; and a recombinant vector which is prepared by inserting the genes encoding DavT and DavD into the expression vector. |
