MEASURING METHOD OF THE SKIN REGENERATION EFFECT USING SKIN EQUIVALENT

摘要

PURPOSE: A measuring method of a skin regeneration effect using a skin equivalent is provided to directly evaluate a skin regeneration effect of a material by performing a wound treatment analysis using a punch biopsy. CONSTITUTION: A dermis layer of a skin regeneration comprises an inside dermis layer and an outside dermis layer. The inside dermis layer comprises a collagen and a fibroblast. The outside dermis layer comprises a collagen. The skin equivalent suppresses a contraction of the dermis. A measuring method of a skin regeneration effect comprises the following steps: a step of making a wound in a epidermis layer of a skin equivalent; a step in which a testing material is processed in a generated wound for 5-10 days; and a step of observing an epimorphic regeneration state of the skin equivalent with time with performing H&E stain(Hematoxylin and Eosin stain) at 2-3 days intervals after processing the testing material. [Reference numerals] (AA) Epidermis layer; (BB) Damaged portion of epidermis; (CC) Dermis layer