摘要 |
Disclosed is a method for purifying leukocytes, the method comprising (a) passing a biological fluid comprising leukocytes, or a cryoprotectant fluid comprising leukocytes, through a porous leukocyte depletion medium having an upstream surface and a downstream surface, the fluid passing from the upstream surface through the downstream surface, wherein leukocytes are retained by the leukocyte depletion medium; (b) passing a wash fluid through the leukocyte depletion medium from the downstream surface through the upstream surface; and, (c) passing the wash fluid through the leukocyte depletion medium from the upstream surface through the downstream surface via gravity.
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