发明名称 |
METHOD FOR PREPARING PROTEIN IMPRINTED POLYMERS AND USE THEREOF |
摘要 |
Methods for preparation of molecularly imprinted polymers and their use for detection of proteins and/or polypeptides in a sample are disclosed. The methods of preparation are based on selecting from available data bases an amino acid sequence of a protein/polypeptide target molecule; cleaving the sequence in-silico with at least one cleaving agent, producing fragments with known composition; selecting at least one such fragment comprising a unique epitope; preparing a synthetic peptide representing the unique epitope; and preparing a molecularly imprinted polymer comprising specific binding sites for the synthetic peptide. For detection of the target protein in a sample, the same cleaving agent used for the in-silico cleavage is used to cleave the target protein to form the specific peptide fragments to which the MIP is specific.
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申请公布号 |
US2013137117(A1) |
申请公布日期 |
2013.05.30 |
申请号 |
US201013386426 |
申请日期 |
2010.07.18 |
申请人 |
LEVI RAPHAEL;MARGALIT IDO;LAUB ORGAD;DLOOMY YARDEN |
发明人 |
LEVI RAPHAEL;MARGALIT IDO;LAUB ORGAD;DLOOMY YARDEN |
分类号 |
G01N33/53 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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