SOLID-PHASE CLONAL AMPLIFICATION AND RELATED METHODS
摘要
The present invention provides methods and compositions for analyzing nucleic acid sequences. In some aspects, the methods utilize clonal objects, such as DNA balls, that have been captured on beads. Using the methods described here, compositions are fabricated wherein a bead and one clonal object are affinity bound or hybridized to each other through an affinity binding patch or hybridization patch on the surface of the bead. The invention also provides a population of beads having affinity bound or hybridized clonal objects at a ratio of 1:1. The invention additionally provides methods for amplifying a target nucleic acid molecule utilizing the compositions described herein.
申请公布号
WO2011123246(A3)
申请公布日期
2013.05.30
申请号
WO2011US28644
申请日期
2011.03.16
申请人
ILLUMINA, INC.;STRAUS, NEIL, A.;SHENGRONG, LIN;ELTHOUKHY, HELMY, A.;GUNDERSON, KEVIN
发明人
STRAUS, NEIL, A.;SHENGRONG, LIN;ELTHOUKHY, HELMY, A.;GUNDERSON, KEVIN