| 摘要 |
PURPOSE: A microdevice for detecting pathogens is provided to detect a pathogen from clinical and environmental samples by sample-in-answer-out capability, and to be applied in biostability test, environmental screening, and clinical test with quick analysis. CONSTITUTION: A microdevice for detecting pathogens comprises: a passive micromixer, a magnetic separation chamber, and a capillary electrophoresis channel. The passive micromixer is used for mixing pathogens, a first probe, and a second probe. The magnetic separation chamber is connected to the passive micromixer. The capillary electrophoresis channel is connected to the magnetic separation chamber. The first probe contains an MMP(magnetic microparticle) probe. The second probe contains nanoparticles of gold, silver, platinum, palladium, copper, nickel, zinc, silicon oxide, or polystyrene. [Reference numerals] (AA) Magnetic field; (BB) Barcode DNA; (CC) Magnetic separation & barcode DNA emission; (DD) Sample; (EE) Separation chamber; (FF) Cathode; (GG) Waste; (HH) Target pathogen capturing through a particle probe; (II) Inlet; (JJ) Manual mixer; (KK) CE channel; (LL) Pathogen detection; (MM) Anode; (NN) Particle probe; (OO) Pathogen; (PP) MMP with the surface on which an antibody is immobilized; (QQ) Magnet; (RR) AuNP modified with FAM tagged barcode DNA and antibody
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