A RECOMBINANT ANTIBODY FOR DIAGNOSIS OF THE PORCINE EPIDEMIC DIARRHEA VIRUS AND THEIR METHOD

摘要

PURPOSE: A recombinant antibody for diagnosing porcine epidemic diarrhea virus is provided to easily maintain or store an antibody producing strain. CONSTITUTION: A method for preparing a recombinant antibody for diagnosing porcine epidemic diarrhea virus comprises: a step of preparing a recombinant baculovirus vector containing a DNA fragment of sequence number 1; a step of transfecting baculovirus full length DNA Bac-N-Blue and the recombinant baculovirus vector into an insect cell and preparing recombinant baculovirus; and a step of culturing the recombinant baculovirus in the insect cell. The vector is pBlueBac4.5His. The insect cell is sf-9 cell.