METHOD FOR INTRODUCTION OF REPLICATIVE AND INTEGRATIVE PLASMIDS INTO THE STRAINS OF AMYCOLATOPSIS JAPONICA DSM44213 AND AMYCOLATOPSIS SP. LV42-5

摘要

A method for introduction of replicative and integrative plasmids into the strains of Amycolatopsis japonica DSM44213 and Amycolatopsis sp. Lv42-5, based on conjugative DNA transfer into Amycolatopsis japonicum MG417-CF17. Amycolatopsis japonica DSM44213 or Amycolatopsis sp. Lv42-5 are used as recipients, and strains of E.coli with plasmid pKC1139 or pSET152 – as donors. At that conjugative mixtures are inoculated into oat medium.