发明名称 |
METHOD FOR GENERATING INDUCED PLURIPOTENT STEM CELLS FROM KERATINOCYTES DERIVED FROM PLUCKED HAIR FOLLICLES |
摘要 |
A method for generating induced pluripotent stem (iPS) cells from isolated hair follicles is disclosed. The method comprises: a. culturing isolated hair follicle keratinocytes on a layer of feeder cells, so as to generate colonies of hair follicle keratinocytes; b. detaching the colonies of hair follicle keratinocytes from the feeder cells so as to generate detached keratinocytes; c. infecting the detached keratinocytes with a virus comprising a nucleic acid molecule encoding at least one dedifferentiation factor so as to generated infected keratinocytes; and d. culturing the infected keratinocytes on a layer of feeder cells in a culture medium until iPS cells are formed, thereby generating iPS cells. Populations and uses of the iPS cells are also disclosed.
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申请公布号 |
US2013130387(A1) |
申请公布日期 |
2013.05.23 |
申请号 |
US201113812566 |
申请日期 |
2011.07.27 |
申请人 |
ITSKOVITZ-ELDOR JOSEPH;NOVAK-PETRARO ATARA;SHTRICHMAN RONIT;TECHNION RESEARCH & DEVELOPMENT FOUNDATION LIMITED |
发明人 |
ITSKOVITZ-ELDOR JOSEPH;NOVAK-PETRARO ATARA;SHTRICHMAN RONIT |
分类号 |
C12N15/85 |
主分类号 |
C12N15/85 |
代理机构 |
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主权项 |
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地址 |
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