| 发明名称 |
Method for Reducing Star Activity in Restriction Endonucleases |
| 摘要 |
Methods are provided for making restriction endonucleases with reduced star activity by one or more targeted mutations to a catalytic site within the restriction endonuclease. Examples of modifications to restriction endonucleases with significant sequence identity with KpnI are provided and reduced star activity demonstrated.
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| 申请公布号 |
US2013130293(A1) |
申请公布日期 |
2013.05.23 |
| 申请号 |
US201213346798 |
申请日期 |
2012.01.10 |
| 申请人 |
NAGARAJA VALAKUNJA;SARAVANAN MATHESHWARAN;ZHU ZHENYU;NEW ENGLAND BIOLABS, INC. |
发明人 |
NAGARAJA VALAKUNJA;SARAVANAN MATHESHWARAN;ZHU ZHENYU |
| 分类号 |
C12N9/16 |
主分类号 |
C12N9/16 |
| 代理机构 |
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