LIGAND HAVING THREE-FINGER STRUCTURE, AND METHOD FOR DETECTING MOLECULE USING SAME

摘要

The purpose of the present invention is to improve the IVV (in vitro virus) synthesis efficiency and the stability of mRNA derived from screened cDNA in a cDNA display method to thereby improve the efficiency and reliability of the production of a peptide by a molecular evolutionary engineering technique. Provided is a ligand which comprises three fingers formed from antiparallel beta-sheets and a loop region intercalated between the antiparallel beta-sheets, wherein at least a fingertip part formed by the loop region of each of the fingers is bound to the target molecule, and wherein the ligand comprises the amino acid sequence represented by SEQ ID NO: 1 shown in the Sequence Listing. In the amino acid sequence represented by SEQ ID NO: 1, X7 represents an arbitrary amino acid residue that constitutes the fingertip part of each of the fingers, each numeric character represents the number of amino acid residues, and X7 and X4 are not composed of the same amino acid residues as each other.