发明名称 |
AZIRIDINE MEDIATED NATIVE CHEMICAL LIGATION |
摘要 |
Improved methods of native chemical ligation are provided. The methods involve reacting a thioacid (e.g. a peptide thioacid) with an aziridinyl compound (e.g. an aziridinyl peptide) under mild conditions without the use of protecting groups, and without requiring that a cysteine residue be present in the ligation product. Initial coupling of the thioacid and the aziridinyl compound yields a ligation product which contains an aziridinyl ring. Subsequent opening of the aziridinyl ring (e.g. via a nucleophilic attack) produces a linearized and modified ligation product.
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申请公布号 |
US2013131311(A1) |
申请公布日期 |
2013.05.23 |
申请号 |
US201213675524 |
申请日期 |
2012.11.13 |
申请人 |
WASHINGTON STATE UNIVERSITY;WASHINGTON STATE UNIVERSITY |
发明人 |
GARNER PHILIP |
分类号 |
C07K1/00;C07K5/062;C07K5/083;C07K5/103;C07K7/06;C07K7/64;C07K9/00;C07K14/00 |
主分类号 |
C07K1/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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