发明名称 |
Cultured cell construction containing spheroids of mesenchymal stem cells and utilization thereof |
摘要 |
Facing no ethical obstacle and easily to be isolated, multipotent concretely mesenchymal stem cells (MSCs) are one of the most powerful tools in reconstructive medicine. Here the inventors introduced 3D multicelluar spheroids culture construction based on photolithography and micropatterning techniques to improve multipotent differentiation efficiency of MSCs to adult cells. This invention, the 3D spheroid cultured construction for MSCs, leads to great improve of the differentiation efficiency. This invention relates to a cultured cell construct comprising a support; at least one island on the support; a hydrophilic and cytophobic substance applied on the surface of said support so as to encircle the island; and a spheroid which is derived from MSCs, said spheroid being in contact with the island.
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申请公布号 |
US8445279(B2) |
申请公布日期 |
2013.05.21 |
申请号 |
US20080255342 |
申请日期 |
2008.10.21 |
申请人 |
WANG WENJIE;ITAKA KEIJI;OHBA SHINSUKE;NISHIYAMA NOBUHIRO;CHUNG UNG-IL;YAMASAKI YUICHI;KATAOKA KAZUNORI;TRANSPARENT INC. |
发明人 |
WANG WENJIE;ITAKA KEIJI;OHBA SHINSUKE;NISHIYAMA NOBUHIRO;CHUNG UNG-IL;YAMASAKI YUICHI;KATAOKA KAZUNORI |
分类号 |
C12N5/00;C12N5/02;C12N5/071 |
主分类号 |
C12N5/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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