| 摘要 |
FIELD: medicine, pharmaceutics.SUBSTANCE: invention refers to pharmacology and medicine, and represents a method for preparing a concentrated culture fluid of active anamorphic fungi species P. farinosus, S. militare, V. brongniartii with antibiotic activity, including growth of the fungi Cordyceps in liquid nutrient media (Czapek's medium, cereal infusion, unhopped wort (7%)) in the steady-state environment followed by concentrating of the culture fluid, characterised be the fact that test insects are further infected intra-hemocoel by G. mellonella (introduction of the suspension 0.01 mL into a hemocoel), immersion of larvae into the suspension for 30 seconds, infection of feed with the fungi Cordyceps for preparing the anamorphic virulent forms; and then biotechnological cultures the concentrated culture medium of which has high antibiotic activity is prepared from the mummified and melanised larvae G. mellonella by fungi re-inoculation from the insects on the liquid nutrient media.EFFECT: invention provides unifying the process of the concentrated culture fluid of the anamorphic fungi Cordyceps.3 ex, 3 tbl |
