BIOENGINEERING STRAIN FOR PRODUCING NEW MICROBIAL FUNGICIDE AND USE THEREOF

摘要

Provided is a bioengineering strain for producing a new microbial fungicide and the use thereof, wherein the bioengineering strain is obtained by transforming a phzH gene recombinant expression plasmid into a strain producing phenazine-1-carboxylic acid. After the recombinant expression plasmid carrying the phzH gene is transformed into the strain producing phenazine-1-carboxylic acid, highly efficient expression of the phzH gene is realized and phenazine-1-carboxylic acid is transformed into phenazine-1-carboxylic acid amide. Therefore, the bioengineering stain can produce phenazine-1-carboxylic acid amide. Also disclosed are the use of the bioengineering stain, comprising producing a microbial fungicide by fermentation, and the preparation method and use of the fungicide. The antifungal activity of phenazine-1-carboxylic acid amide is not affected by the acidity value of the conditions of use, thereby having a more stable antifungal activity.